Last updated: 2021-11-16

Checks: 1 1

Knit directory: MS_lesions/

This reproducible R Markdown analysis was created with workflowr (version 1.6.2). The Checks tab describes the reproducibility checks that were applied when the results were created. The Past versions tab lists the development history.


The R Markdown file has unstaged changes. To know which version of the R Markdown file created these results, you’ll want to first commit it to the Git repo. If you’re still working on the analysis, you can ignore this warning. When you’re finished, you can run wflow_publish to commit the R Markdown file and build the HTML.

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The results in this page were generated with repository version 8d1ece6. See the Past versions tab to see a history of the changes made to the R Markdown and HTML files.

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Ignored files:
    Ignored:    .DS_Store
    Ignored:    .Rhistory
    Ignored:    .Rprofile
    Ignored:    .Rproj.user/
    Ignored:    ._MS_lesions.sublime-project
    Ignored:    .log/
    Ignored:    MS_lesions.sublime-project
    Ignored:    MS_lesions.sublime-workspace
    Ignored:    analysis/.__site.yml
    Ignored:    analysis/fig_muscat_cache/
    Ignored:    analysis/ms02_doublet_id_cache/
    Ignored:    analysis/ms03_SampleQC_cache/
    Ignored:    analysis/ms04_conos_cache/
    Ignored:    analysis/ms05_splitting_cache/
    Ignored:    analysis/ms06_sccaf_cache/
    Ignored:    analysis/ms07_soup_cache/
    Ignored:    analysis/ms08_modules_cache/
    Ignored:    analysis/ms08_modules_pseudobulk_cache/
    Ignored:    analysis/ms09_ancombc_cache/
    Ignored:    analysis/ms09_ancombc_clean_1e3_cache/
    Ignored:    analysis/ms09_ancombc_clean_2e3_cache/
    Ignored:    analysis/ms09_ancombc_mixed_cache/
    Ignored:    analysis/ms10_muscat_run01_cache/
    Ignored:    analysis/ms10_muscat_run02_cache/
    Ignored:    analysis/ms10_muscat_template_broad_cache/
    Ignored:    analysis/ms10_muscat_template_fine_cache/
    Ignored:    analysis/ms11_paga_cache/
    Ignored:    analysis/ms12_markers_cache/
    Ignored:    analysis/ms13_labelling_cache/
    Ignored:    analysis/ms14_lesions_cache/
    Ignored:    analysis/ms15_mofa_sample_gm_cache/
    Ignored:    analysis/ms15_mofa_sample_gm_final_meta_cache/
    Ignored:    analysis/ms15_mofa_sample_gm_superclean_cache/
    Ignored:    analysis/ms15_mofa_sample_wm_cache/
    Ignored:    analysis/ms15_mofa_sample_wm_final_meta_cache/
    Ignored:    analysis/ms15_mofa_sample_wm_new_meta_cache/
    Ignored:    analysis/ms15_mofa_sample_wm_superclean_cache/
    Ignored:    analysis/ms15_patients_cache/
    Ignored:    analysis/ms15_patients_gm_cache/
    Ignored:    analysis/ms15_patients_sample_level_cache/
    Ignored:    analysis/ms15_patients_w_ms_cache/
    Ignored:    analysis/supp06_sccaf_cache/
    Ignored:    analysis/supp07_superclean_check_cache/
    Ignored:    analysis/supp09_ancombc_cache/
    Ignored:    analysis/supp09_ancombc_mixed_cache/
    Ignored:    analysis/supp09_ancombc_superclean_cache/
    Ignored:    analysis/supp10_muscat_cache/
    Ignored:    analysis/supp10_muscat_ctrl_gm_vs_wm_cache/
    Ignored:    analysis/supp10_muscat_heatmaps_cache/
    Ignored:    analysis/supp10_muscat_olg_pc1_cache/
    Ignored:    analysis/supp10_muscat_olg_pc2_cache/
    Ignored:    analysis/supp10_muscat_olg_pc_cache/
    Ignored:    analysis/supp10_muscat_regression_cache/
    Ignored:    analysis/supp10_muscat_soup_cache/
    Ignored:    analysis/supp10_muscat_soup_mito_cache/
    Ignored:    code/._ms10_muscat_fns_recover.R
    Ignored:    code/.recovery/
    Ignored:    code/jobs/._muscat_run09_2021-10-11.slurm
    Ignored:    code/muscat_plan.txt
    Ignored:    data/
    Ignored:    figures/
    Ignored:    output/
    Ignored:    tmp/

Untracked files:
    Untracked:  analysis/supp09_ancombc_superclean.Rmd
    Untracked:  analysis/supp10_muscat_soup_mito.Rmd
    Untracked:  analysis/supp99_pseudosoup.Rmd
    Untracked:  code/ancom_v2.1.R
    Untracked:  code/dev_check_eqtls_against_groupings_2021-10-15.R
    Untracked:  code/dev_check_genotyping_20211007.R
    Untracked:  code/dev_check_mt_in_soup_2021-10-18.R
    Untracked:  code/dev_de_w_contamation_2021-10-25.R
    Untracked:  code/glmmtmb_code_for_julien_2021-10-13.R
    Untracked:  code/jobs/muscat_run09_2021-10-11.slurm
    Untracked:  code/jobs/muscat_run09_2021-10-12.slurm
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    Untracked:  code/jobs/muscat_run09_2021-10-14.slurm
    Untracked:  code/jobs/muscat_run10_2021-10-11.slurm
    Untracked:  code/jobs/muscat_run10_2021-10-12.slurm
    Untracked:  code/jobs/muscat_run10_2021-10-13.slurm
    Untracked:  code/jobs/muscat_run11_2021-10-11.slurm
    Untracked:  code/jobs/muscat_run11_2021-10-14.slurm
    Untracked:  code/jobs/muscat_run11_2021-10-21.slurm
    Untracked:  code/jobs/muscat_run12_2021-10-11.slurm
    Untracked:  code/jobs/muscat_run12_2021-10-14.slurm
    Untracked:  code/jobs/muscat_run12_2021-10-21.slurm
    Untracked:  code/jobs/muscat_run19_2021-10-14.slurm
    Untracked:  code/jobs/muscat_run23_2021-11-15.slurm
    Untracked:  code/jobs/run_ancombc_bootstraps_2021-11-16.slurm
    Untracked:  code/metadata_update_plan_20210928.txt
    Untracked:  code/ms10_muscat_fns_recover.R
    Untracked:  code/ms10_slurm_jobs.py
    Untracked:  code/muscat_slurm_template.slurm
    Untracked:  code/negbin_std.stan
    Untracked:  code/negbin_w_contam.stan
    Untracked:  code/negbin_w_contam_v2.stan
    Untracked:  code/run_ancombc_bootstraps_2021-11-16.R

Unstaged changes:
    Modified:   analysis/fig_muscat.Rmd
    Modified:   analysis/index.Rmd
    Modified:   analysis/ms07_soup.Rmd
    Modified:   analysis/ms14_lesions.Rmd
    Modified:   analysis/ms15_mofa_sample_wm_superclean.Rmd
    Modified:   analysis/supp07_superclean_check.Rmd
    Modified:   analysis/supp09_ancombc.Rmd
    Modified:   analysis/supp10_muscat_soup.Rmd
    Deleted:    code/dev_checking_hacked_pb_2021-07-16.R
    Modified:   code/fig_muscat.R
    Modified:   code/jobs/muscat_job_2021-10-04_fine_gm.slurm
    Modified:   code/jobs/muscat_job_2021-10-04_fine_wm.slurm
    Modified:   code/ms07_soup.R
    Modified:   code/ms10_muscat_fns.R
    Modified:   code/ms10_muscat_runs.R
    Modified:   code/ms14_lesions.R
    Modified:   code/supp10_muscat.R

Staged changes:
    Modified:   analysis/ms09_ancombc_mixed.Rmd
    Modified:   code/ms09_ancombc_mixed.R

Note that any generated files, e.g. HTML, png, CSS, etc., are not included in this status report because it is ok for generated content to have uncommitted changes.


These are the previous versions of the repository in which changes were made to the R Markdown (analysis/index.Rmd) and HTML (docs/index.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.

File Version Author Date Message
Rmd b9e43a8 Macnair 2021-10-23 Update index
html b9e43a8 Macnair 2021-10-23 Update index
Rmd 8a15881 Macnair 2021-10-18 Update index.Rmd with final MOFA results</td> </tr> <tr> <td>html</td> <td><a href="https://rawcdn.githack.com/wmacnair/MS_lesions/8a15881721c1904566ddafacc5e6e0e06412bd92/docs/index.html" target="_blank">8a15881</a></td> <td>Macnair</td> <td>2021-10-18</td> <td>Update index.Rmd with final MOFA results
Rmd 1552617 Macnair 2021-10-05 Save MOFA genes to xls
html 1552617 Macnair 2021-10-05 Save MOFA genes to xls
Rmd c1a0bff Macnair 2021-10-05 Update GM MOFA with final metadata
html c1a0bff Macnair 2021-10-05 Update GM MOFA with final metadata
Rmd c679b20 Macnair 2021-10-04 Update index
html c679b20 Macnair 2021-10-04 Update index
Rmd 391aef9 Macnair 2021-10-04 Update index.html with new mofa analysis
html 391aef9 Macnair 2021-10-04 Update index.html with new mofa analysis
html 62b9816 Macnair 2021-09-24 Update index.html
Rmd 9153b78 Macnair 2021-09-24 Update index.Rmd
Rmd dc248fa Macnair 2021-09-03 Update index.html with links
html dc248fa Macnair 2021-09-03 Update index.html with links
Rmd 96d385e Macnair 2021-08-24 Update index.html, also a couple of minor tweaks
html 96d385e Macnair 2021-08-24 Update index.html, also a couple of minor tweaks
Rmd f60e5c1 Macnair 2021-08-03 Add astrocyte layer genes to ms12_markers
html f60e5c1 Macnair 2021-08-03 Add astrocyte layer genes to ms12_markers
Rmd 47b429a Macnair 2021-07-23 Analyse ctrl GM vs WM, fine celltypes
html 47b429a Macnair 2021-07-23 Analyse ctrl GM vs WM, fine celltypes
Rmd 903c384 Macnair 2021-07-22 Update MOFA analysis
html 903c384 Macnair 2021-07-22 Update MOFA analysis
Rmd 3a29e55 Macnair 2021-07-09 Add MOFA patient analysis (WM only)
html 3a29e55 Macnair 2021-07-09 Add MOFA patient analysis (WM only)
Rmd 1036875 Macnair 2021-07-02 Add analysis of oligo-PCs via muscat
html 1036875 Macnair 2021-07-02 Add analysis of oligo-PCs via muscat
Rmd 0ec42e1 Macnair 2021-06-20 Update GSEA analysis, heatmaps, lesion-specific
html 0ec42e1 Macnair 2021-06-20 Update GSEA analysis, heatmaps, lesion-specific
Rmd 856df8d Macnair 2021-06-15 Update index with changes
html 856df8d Macnair 2021-06-15 Update index with changes
Rmd 8ccd4e7 Macnair 2021-06-08 Update muscat reports
html 8ccd4e7 Macnair 2021-06-08 Update muscat reports
Rmd 5ab29c3 Macnair 2021-06-03 typo in index.html
html 5ab29c3 Macnair 2021-06-03 typo in index.html
Rmd bb0eebf Macnair 2021-06-03 Updated index.html
html bb0eebf Macnair 2021-06-03 Updated index.html
Rmd cfacffb Macnair 2021-05-27 Updated index.html
html cfacffb Macnair 2021-05-27 Updated index.html
Rmd 7f4198b Macnair 2021-05-26 Updated index file
html 7f4198b Macnair 2021-05-26 Updated index file
Rmd d4b022a Macnair 2021-05-21 Fix typo in index.Rmd
html d4b022a Macnair 2021-05-21 Fix typo in index.Rmd
Rmd 58205c2 Macnair 2021-05-21 Update with random effects and markers
html 58205c2 Macnair 2021-05-21 Update with random effects and markers
html 9852840 Macnair 2021-05-12 Adding docs to repo for first time - massive!
Rmd eef8a1c Macnair 2021-04-29 Minor tweaks to allow rerunning on Roche servers
Rmd a6221cc wmacnair 2021-04-07 Giant update of recent work
Rmd d2a3b50 wmacnair 2021-02-10 Added admin files
Rmd 31fc587 wmacnair 2021-01-19 Initial commit of mainly blank repo

Overview

Pre-processing

Checks / additional analysis (not currently intended to be included in the paper)

These are generally checks against what we had before Christmas.